College of Forestry

Forest Biotechnology Laboratory

Genetic transformation of hops


With support from USDA, we are working to develop gene-editing and transformation (gene transfer) systems in North American hop varieties to aid breeding for key traits, such as disease resistance, flavor quality, and flowering. Part of this work is to customize regeneration (cell to plant differentiation) for individual high value clones, such as for Cascade as shown below. Of the different cytokinins (shoot-inducing hormones) tested in Cascade, high levels of BAP were most effective at inducing new shoots in tissue culture.

Bar graph and photos: Cascade de novo shoot generation after 8 weeks.

Presentations

Development of Gene Editing Methods to Retain Access to Foreign Markets for American Hops
Hop Research Council Annual Meeting, Oregon State University, Corvallis. August 2024. 
C. Willig

Potential for CRISPR/gene-editing in hop breeding
Master Brewers National Meeting, Seattle, WA, October 2023
Strauss, SH and C Willing

Gene editing as a powerful tool to advance hop research and agriculture 
Hop Research Council Meeting, August 2023, Boise, ID 
Chris Willig

Toward Gene Editing in Hop: Application of a Cutting-Edge Tool for Accelerating Breeding and Improving Traits 
Hop Research Council Meeting, January 2023, Santa Rosa, CA 
Chris Willig

“Gene Editing for Brewers”    
Master Brewers Podcast, October 2022    
Professors Steve Strauss and Tom Shellhammer, Oregon State University

Genetic modification methods: Basics and application to hops    
Brewing Summit 2022 (ASBC​ a​nd Master Brew​er​s joint meeting), Convention Center, Providence, Rhode Island, August 2022    
Steve Strauss, Chris Willig, Michele Wiseman, David Gent, John Henning, and Tom Shellhammer

Gene Editing in Hops: Methods, Possibilities, and Progress    
Poster presented at: Brewing Summit 2022 (ASBC​ a​nd Master Brew​er​s joint meeting), Convention Center, Providence, Rhode Island, August 2022    
Christopher J. Willig, Michele S. Wiseman, John A. Henning, David H. Gent, Tom Shellhammer, and Steven H. Strauss